Associate Professor (September 2015 - till date), Biological Sciences, IISER Bhopal, India.
Assistant Professor (June 2009 - August 2015), Biological Sciences, IISER Bhopal, India.
Ph.D. in (Biological Science) 2006 from Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
The MMBL group at IISER Bhopal is actively involved in both basic and applied research in bacteriophages. In particular, we are studying the process of phage infection of mycobacteria and the downstream activities that occur inside the cell post-infection. Several proteins that are involved in transcription of phage late genes and the release of mature phage particles are of specific interest in addressing questions related to their regulation and future application in phage-based therapy for tuberculosis infection. Additionally, we are also looking into the bacterial perspective, in terms of tolerance development, resistance to phage infection and evolution of such phenotype. We have developed a combinatorial approach to understand the phage-host system that utilizes both basic and advanced research tools available in Biophysics, Biochemistry, Molecular Biology and Microbiology to address specific questions such as the process of phage infection, phage gene transcription, virion release, bacterial cell injury and recovery, cell death and cell survival strategies. We employ spectroscopic methods, including circular dichroism, steady state and time resolved fluorescence coupled with 2D proteomics and mass spectrometric approaches, as well as biophysical and modern microscopic imaging to gain novel insight into the unique interaction that has evolved between mycobacteriophage and its host.
Singh, MI, Ganesh, B, Jain, V. On the domains of T4 phage sliding clamp gp45: an intermolecular crosstalk governs structural stability and biological activity. Biochimica et Biophysica Acta 2017, 1861: 3300-3310. (Pubmed)
Dubey, AA, Singh, MI, Jain, V. Rapid and robust PCR-based all-recombinant cloning methodology. PLoS One 2016, 11(3): e0152106. (Pubmed)
Singh, MI, Jain, V. Molecular dissection of the homotrimeric sliding clamp of T4 phage: Two domains of a subunit display asymmetric characteristics. Biochemistry 2016, 55(3): 588-596. (Pubmed)
Lella, M, Kamilla, S, Jain, V, Mahalakshmi, R. Molecular mechanism of holin transmembrane domain I in pore formation and bacterial cell death. ACS Chem. Biol. 2016, 11: 910-920. (Pubmed)
Kamilla, S, Jain, V. Mycobacteriophage D29 holin C-terminal region functionally assists in holin aggregation and bacterial cell death. FEBS J. 2016, 283(1): 173-190. (Pubmed)
Pohane, AA, Jain, V. Insights into the regulation of bacteriophage endolysin: Multiple means to the same end. Microbiology 2015, 161: 2269-2276. (Pubmed)
Syal, K, Joshi, H, Chatterji, D, Jain, V. Novel pppGpp binding site at the C-terminal region of Rel enzyme from Mycobacterium smegmatis. FEBS J. 2015, 282(19): 3773-3785. (Pubmed)
Pohane, AA, Patidar, ND, Jain, V. Modulation of domain-domain interaction and protein's function by a charged linker: A case study of mycobacteriophage D29 endolysin. FEBS Lett. 2015, 589(6): 695-701. (Pubmed)
Sharma, A, Pohane, AA, Bansal, S, Bajaj, A, Jain, V, Srivastava, A. Cell Penetrating Synthetic Antimicrobial Peptides (SAMPs) Exhibiting Potent and Selective Killing of Mycobacterium by Targeting Its DNA. Chem. Eur. J. 2015, 21(9): 3540-3545. (Pubmed)
Ekal, L, Ganesh, B, Joshi, H, Lama, D, Jain V. Evidence of a conserved intrinsically disordered region in the C-terminus of the stringent response protein Rel from mycobacteria. FEBS Lett. 2014, 588(9): 1839-1849. (Pubmed)
Pohane, AA, Joshi, H, Jain, V. Molecular dissection of phage endolysin: An interdomain interaction confers host specificity in Lysin A of Mycobacterium phage D29. J. Biol. Chem. 2014, 289(17): 12085-12095. (Pubmed)
Singh, MI and Jain, V. Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options. PLoS ONE 2013, 8(5): e63922. (Pubmed)
Twist, KA, Campbell, EA, Deighan, P, Nechaev, S, Jain, V, Geiduschek, EP, Hochschild, A and Darst, SA. Crystal structure of the bacteriophage T4 late-transcription co-activator gp33 with the β-subunit flap domain of E. coli RNA polymerase. Proc. Natl. Acad. Sci. USA 2011, 108(50): 19961-19966. (Pubmed)
Jain, V, Saleem-Batcha, R, Chatterji, D. Synthesis and hydrolysis of pppGpp in mycobacteria: a ligand mediated conformational switch in Rel. Biophys. Chem. 2007, 127(1-2): 41-50. (Pubmed)
Jain, V, Saleem-Batcha, R, China, A, Chatterji, D. Molecular dissection of the mycobacterial stringent response protein Rel. Protein Sci. 2006, 15(6): 1449-1464. (Pubmed)
Jain, V, Kumar, M and Chatterji, D. ppGpp: Stringent response and survival. J. Microbiol. 2006, 44(1): 1-10. (Pubmed)
Jain, V, Sujatha, S, Ojha, AK, Chatterji, D. Identification and characterization of rel promoter element of Mycobacterium tuberculosis. Gene 2005, 351: 149-157. (Pubmed)
Sivakumar, P, Sharmila, P, Jain, V and Saradhi, PP. Sugars have potential to curtail oxygenase activity of Rubisco. Biochem. Biophys. Res. Commun. 2002, 298: 247-250. (Pubmed)
Mr. Himanshu Joshi
Ms. Manika I. Singh
Ms. Minakshi Nandanwar
Mr. Abhishek Anil Dubey
Mr. Surya Pratap Seniya
Mr. Varun Rakeshbhai Bavda
Mr. Srashti Gopal Goyal
Ms. Varsha Patil
Ms. Dilsha C.
Ms. Anu Negi
Mr. Mohit K. Manhas