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Central Instrumentation Facility

NANO-LC MALDI TOF/TOF SPECTROMETER

A brand new MALDI TOF spectrometer has been installed in the CIF mass facility. Combining true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh performance and extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications. The smartbeam laser provides unprecedented analytical and matrix flexibility in workflows from protein tissue imaging, intact proteins analysis, Glycoproteomics, biologics or oligo QC, to LC-MALDI proteomics - all fully enabled at 1-2000 Hz repetition rates. The high efficiency and sensitivity of the LID-LIFT process delivers MS/MS spectra with nominal mass resolution for peptides. Typically, full MS/MS data sets can be acquired with up to 1000 Hz laser repetition rate from low fmol levels within seconds. The software is perfectly adapted to analyze and visualize the match between the raw spectra and annotated peptide and protein structures. The offline coupling of liquid chromatography (LC) to MALDI mass spectrometry (LC-MALDI) has been shown to be a relevant technique in proteomics, comprising protein profiling and protein identification capabilities. Major advantages are the reduction of complexity and selection of precursor ions for the MS/MS data acquisition without any time constrains. The PROTEINEER fc II enables automatic liquid handling for MALDI preparation of LC separated peptide fractions. It automates the Proteomics workflow from LC-separation to mass spectrometric identification and characterization by MALDI TOF and TOF/TOF systems.

HRMS

A Bruker microTOF QII high-resolution mass spectrometer is coupled to Waters Acquity UPLC system. The instrument is capable of analysing most type of samples including solid samples.

Dr. Debasish Manna

Instrument In-charge

dmanna[[AT]iiserb.ac.in
| +91 755 269 1359 |

Ms. Ruchi Shrivastava

Project Lab Assistant

ruchis[AT]iiserb.ac.in
| +91 755 269 2337 |

Mr. Brijesh Sharma

Project Lab Assistant

brijeshk[AT]iiserb.ac.in
| +91 755 269 2337 |

GCMS

The GC-MS system from Agilent is one of the best instruments available in the category. It comprises of Agilent 7890A GC with Agilent 5975C MS with a dual detector mode (FID and MS together). The MS is a single quadrupole system with mass range up to 1040 u with synchronous SIM/Scan. The MS ionization can be switched between EI and CI modes. With the EI mode, ionization up to 240 eV is possible.

GC-QTOF And LC-Single Quadrupole

The other two instruments in the mass facility include GC-QTOF and LC-single quadrupole spectrometer from Agilent. The GC-QTOF is capable of providing HRMS for various kinds of samples and the LC-Single quadrupole mass spectrometer can handle various types of samples including biological molecules. It includes APCI option as well.

Quadrupole Time of Flight LC/MS 6546 LC/Q-TOF

The 6546 LC/Q-TOF coupled with the 1290 Infinity II LC System is the centerpiece of multiple workflows, allowing uncompromised data quality in qualitative and quantitative analyses. The 6546 LC/Q-TOF accelerates the lab’s capabilities by enabling comprehensive workflows in metabolomics research, food safety, food authenticity, and environmental screening. With simultaneous high sensitivity, wide dynamic range, high resolution, isotope fidelity, and accurate mass measurements, the resulting high data quality provides fast and simplified data review processes.

Sensitivity MS Mode :   >500:1 RMS in 10 replicates
Sensitivity MS/MSMode :   > 1500:1 RMS in 10 replicates
Mass resolving power :   >60,000 FWHM at m/z 2,722
Mass range :   m/z 50–10,000
Accuracy MS Mode :   <0.8 ppm RMS in 10 replicates
Accuracy MS/MSMode :   <2.0 ppm RMS in 10 replicates
Dynamic range (Spectral) :   Up to 5 th Order
Spectral acquisition rate :   50 spectra/second
Isotope fidelity :   Relative isotopic abundance error< 5%
Features :   Swam Autotune, Fragile Ion tuning
Applications :   Metabolomics, Proteomics, Food authenticity testing

Dr. Debasish Manna

Instrument In-charge

dmanna[[AT]iiserb.ac.in
| +91 755 269 1359 |

Ms. Ruchi Shrivastava

Project Lab Assistant

ruchis[AT]iiserb.ac.in
| +91 755 269 2337 |

Mr. Brijesh Sharma

Project Lab Assistant

brijeshk[AT]iiserb.ac.in
| +91 755 269 2337 |

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